Cigarette smoke extract experiments
WebSep 17, 2014 · Abstract. Cigarette smoke consists of tar and gas phase: the latter is toxicologically important because it can pass through lung alveolar epithelium to enter … WebSep 17, 2024 · Here, we investigated the effects of cigarette smoke extract (CSE) and HTPs on the corneal epithelium using an in-vitro cell culture system. CSE treatment perturbed the structure and function...
Cigarette smoke extract experiments
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WebThis experiment will utilize the movement of Tetrahymena to measure the effect of cigarette smoke and alcohol on cilia function. By observing the movement and … WebAbstract. Smoking tobacco is a major risk factor for the development of lung cancer, COPD, and other lung pathologies in smokers. Cigarette smoke (CS), which is …
WebApr 29, 2013 · The experimental approach was to expose A549 cells to 50 μg/ml cigarette smoke extract (dissolved in DMSO), compared to control cells treated with DMSO alone. The positive control cells were exposed to 10 ng/ml IL-1β which is known to up-regulate the expression of many defensin peptides [ 33 ]. WebHello, I'm doing a smoking study on BEAS-2B cells, and was wondering what the most appropriate method of preparing cigarette smoke extract in the lab, I do not have access to a smoke machine. Also ...
WebJan 9, 2024 · For the smoke exposure experiments, cigarette smoke extract (CSE) was prepared as previously described . Beas2b cells were treated with cysteamine and/or CSE at various concentrations for 6 h or overnight. Western blotting. Caspase-3/7, DNA fragmentation, and senescence assays. WebUnder a cigarette smoke extract (CSE) stimulation condition, the apoptosis was analyzed by flow cytometry assay. Caspase-3 activity was examined with a caspase-3 activity assay kit; besides, inflammation and oxidative stress were assessed by measuring interleukin-6, tumor necrosis factor-α, glutathione/oxidized glutathione, reactive oxygen ...
WebMar 31, 2024 · Cigarette smoke extract (CSE) was prepared by bubbling smoke from two cigarettes (1.2 mg nicotine and 14 mg tar) without a filter into 20 mL of serum-free Ham’s F-12 K medium at a rate of 1 cigarette/min as previously described [ 11 ]. The pH of the medium was adjusted to 7.4 using HCl, and the medium was sterile filtered with a 0.22 …
WebNov 6, 2024 · Cigarette smoke extract (CSE) was prepared as described below. In short, two non-filtered cigarettes (containing 0.8 mg of nicotine and 10 mg of tar per cigarette, Shishi, China) were burned with a vacuum pump, and then smoke was bubbled through the 20 ml serum-free medium to collect CSE. sld incorporated tracy caWebIn this study, we demonstrate how a mixture of plant sterols (MPtS) consisting of β-sitosterol, campesterol and stigmasterol, at serum concentration reached after ingestion of a drink … sld intial setupWebJan 1, 2024 · Experiments were repeated using cigarette smoke extract (CSE) for comparison. IL-8 was measured by ELISA, activation of NF-kappaB by ELISA and Western blotting, and caspase-3 activity by Western ... sld incWebMay 5, 2024 · (A,C) Representative Ca 2+ imaging traces from separate experiments tracking changes in [Ca 2+] i following puffing one whole cigarette (A) or following Ca 2+ addback after 5-min perfusion with 10% CSE (C) with or without 10-min pre-treatment with 50 µM HC-030031, which was also present throughout the experiment. Equal volumes … sld industries scamWebOct 18, 2024 · This extract is created by bubbling cigarette smoke through a solution and is often used in preclinical studies as an analogy for the smoke itself. The researchers … sld industries inchttp://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-affects-methylation-status-and-nattenuates-Sca-1-expression,131625,0,2.html sld interiorsWebAug 12, 2024 · Electronic cigarette extract (ECE) was prepared by bubbling smoke from the electronic cigarette using liquid that contains 6 mg of nicotine per ml through 15 mL of deionized water at a speed of 60 cc/min for 10 min. Cigarette smoke extract (CSE) was prepared from one 84-mm cigarette (research cigarette 3R4F; University of Kentucky) … sld investopedia