Jc-1 red/green ratio
WebEvaluation of the JC-1 red/green fluorescence intensity ratio revealed similar levels of mitochondrial membrane potential in cells growing on graphene-coated and uncoated slides. These results indicate that graphene monolayer scaffold is cytocompatible with connective tissue cells examined and could be beneficial for tissue engineering therapy. WebOct 23, 2013 · B, FCCP reduced the MMP in vivo as indicated with the JC-1 ratio (red/green) in Wt mouse cortex. FCCP (10 μ m) was applied topically to the cortex for 15 min. The ratio of J-aggregate (red) to J-monomer (green) changed in …
Jc-1 red/green ratio
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WebJC‐1 does not form aggregates in mitochondria’s with low ∆ψ M and remain in monomeric form and exhibit green fluorescence. Thus higher the ratio of red to green fluorescence, … WebMay 22, 2006 · The aggregated form of JC-1 molecules (red fluorescence) accumulated in functional mitochondria. On the other hand, the green fluorescence signal from JC-1 monomers is evenly distributed in the cytoplasm (Fig. 1 A and B).In the control groups, the red/green ratio was 18.2 ± 9.3, while in the hypoxia/re-oxygenization group this ratio was …
WebSep 1, 2011 · Due to perfect alignment of the two images, we established an online ratiometric analysis of JC-1 fluorescence (ratio = JC-1 red *5/JC-1 green; the factor “5” transformed the ratiometric results into an 8-bit scale of 256 levels). WebJan 26, 2004 · The fluorescent signal intensity at 590 nm (red) was divided by the intensity at 535 nm (green), and this red/green ratio was used to estimate the mitochondrial membrane potential (ΔΨ m ). Larger JC-1 red/green …
WebCayman’s JC-1 Mitochondrial Membrane Potential Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in Δψ M … WebJC-1 and JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red …
WebJC-1 is a green-fluorescent (λ ex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λ em 596 nm ) "J-aggregates," which exhibit broad …
WebSep 1, 2011 · Red JC-1 fluorescence clearly increased up to three-fold, whereas green JC-1 fluorescence showed less intense and opposite changes (see Fig. 4a). Accordingly, these … portal fielsoWebAug 29, 2024 · After 30 s, 1.2 mL of an acridine orange (AO) solution (6 μg/mL in 37 mM citric acid, 126 mM Na 2 HPO 4, 1.1 mM EDTA, 150 mM NaCl, pH = 6.0) was added, and samples were kept in ice for further 3 min. Immediately after this time, samples were evaluated and green and red fluorescence were collected through FL1 and FL3 filters, … irsd preschoolWebCayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in Δψ M, reflected by aggregation level of JC-1, can be determined as a ratio of red to green mean fluorescence intensities using flow cytometry. irsd lunchWebFeb 8, 2024 · To verify that inhibition of mitoNEET decreases the mitochondrial iron content, we stained mitochondrial iron using the mitochondrial probes Mitochondrial Marker Deep Red and Mito-ferroGreen by,... irsd craWebJC-1 染色试剂可用作多种样本包括肌细胞、神经元细胞）以及完整组织和离体线粒体的线粒体电位的指示剂。. 绿色与红色荧光的比例仅取决于线粒体膜电位，与线粒体大小、形状 … portal firstfin creditWebMitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨ m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a ... portal firmy luxmedWebJan 15, 2001 · It was shown that JC-1 (green and red bands) may define 3 groups of patients: resistant (R) (29% of patients), intermediate (I) (36%), and sensitive (S) (35%). In contrast, rhodamine 123 assay detected only the R group defined by JC-1. portal first data